| 用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质 |
| 投稿时间：2014-09-04 修订日期：2014-10-08 点此下载全文 |
| 引用本文：纪松岗,丁璇,曹岩,陈啸飞,贾丹,王冬尧,柴逸峰.用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质[J].药学实践杂志,2014,32(6):425~427,433 |
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| 基金项目:国家自然科学基金青年项目(12401900802). |
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| 中文摘要:目的 筛选中药鸦胆子中的抗癌活性成分。方法 同时使用全二维色谱技术和细胞膜色谱技术联用质谱技术。结果 经初步分离筛选,得到了腺苷和鸦胆子苦素B两种潜在的抗癌活性成分。结论 该方法对中药中活性成分的筛选,具有快速、高效和在线的特色和优点。 |
| 中文关键词:细胞膜色谱 全二维色谱 SMMC-7721 Hep-G2 鸦胆子 抗癌 |
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| Screening of potential active anti-cancer components of Brucea javanica by SMMC-7721 and Hep-G2 comprehensive two dimensional CMC-monolith chromatography |
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| Abstract:Objective To screen potential active anti-cancer components of Brucea javanica.Methods This research has employed comprehensive two dimensional chromatographic technology and cell membrane chromatographic technology simultaneously with mass spectrometry as detector.Results Adenosine and Bruceine B were found to be potentially anti-cancer active.Conclusion This study has combined the advantages of online, high speed and high throughput for the screen of potential active components of traditional Chinese medicine. |
| keywords:cell membrane chromatography comprehensive two dimensional chromatography SMMC-7721 Hep-G2 Brucea javanica anti-cancer |
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