| 应用高效液相色谱法测定葫芦素片中葫芦素B的含量 |
| 投稿时间：2012-11-13 修订日期：2013-04-08 点此下载全文 |
| 引用本文：豆甲泰,李胜文.应用高效液相色谱法测定葫芦素片中葫芦素B的含量[J].药学实践杂志,2014,32(4):294~296 |
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| 中文摘要:目的 测定葫芦素片中葫芦素B的含量。方法 采用高效液相色谱法,应用Waters Symmetry C18(250 mm×4.6 mm,5.0 μm)色谱柱,流动相:乙腈-水(51:49),流速:1.0 ml/min,柱温:25℃,检测波长:228 nm。结果 葫芦素B的回归方程:Y=1 067.3C-0.508 4(r=0.999 9),平均回收率为99.39%(n=6),RSD为0.56%,说明该法具有良好的回收率。结论 用HPLC法测定葫芦素片中葫芦素B的含量,方法简便,重复性好,结果准确。 |
| 中文关键词:HPLC:葫芦素片 葫芦素B |
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| Determination of content of cucurbitacin B in cucurbitacins by HPLC |
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| Abstract:Objective To determina the content of cucurbitacin B in cucurbitacins.Methods The highly effective liquid phase chromatography was used,with Waters Symmetry C18 (250 mm× 4.6 mm 5 μm) column.The mobile phase was acetonitrile-water (51:49), the flow rate was 1 ml/min, column temperature was 25℃, the detection wavelength was 228 nm.Results Cucurbitacin B regression equation: Y=1 067.3C-0.508 4 (r=0.999 9), the average recovery rate was 99.39%(n=6), RSD was 0.56%, which showed that this method had a good recovery rate.Conclusion The HPLC method for the determination of content of cucurbitacin B in cucurbitacins was simple, reproducible, accurate. |
| keywords:HPLC cucurbitacin tablet cucurbitacin B |
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