| 抗肿瘤多肽蜂毒素的固相合成 |
| 投稿时间：2012-12-16 修订日期：2013-08-31 点此下载全文 |
| 引用本文：李文娟,李任武,吴茂诚,王傅喆,秦丽萍,李柏,胡宏岗.抗肿瘤多肽蜂毒素的固相合成[J].药学实践杂志,2014,32(1):51~52 |
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| 基金项目:国家自然科学基金(20902109). |
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| 中文摘要:目的 合成具有抗肿瘤活性的二十六肽蜂毒素。方法 采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/DCC为缩合剂的合成工艺。结果 通过本法能够顺利合成得到二十六肽蜂毒素,收率达32%。结论 该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成。 |
| 中文关键词:蜂毒素 固相合成 逐步缩合 |
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| Solid-phase synthesis of melittin of anti-tumor peptide |
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| Abstract:Objective To synthesize the antitumor activity of twenty-six peptide melittin.Methods The melittin was synthesized by stepwise solid-phase peptide synthesis (SPPS). During the synthesis of melittin, wang-resin was chosen as solid supporter and HOBt/DCC were used as the coupling reagent.Results The melittin was synthesized with yield of 32%. Conclusion The synthetic method was feasible, simple and with high yield for long chain polypeptide synthesis. |
| keywords:melittin solid-phase peptide synthesis stepwise synthesis |
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