| 高效液相色谱法测定伊赫汤中羟基红花黄色素A的含量 |
| 投稿时间：2012-06-25 修订日期：2012-09-19 点此下载全文 |
| 引用本文：莎日娜,何春龙,王焕芸.高效液相色谱法测定伊赫汤中羟基红花黄色素A的含量[J].药学实践杂志,2013,31(2):140~142 |
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| 基金项目:内蒙古自治区高等学校科学研究项目(NJZY12144) |
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| 中文摘要:目的 建立伊赫汤中羟基红花黄色素A的含量测定方法。方法 采用高效液相色谱法(HPLC),色谱柱为大连依力特C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.5%乙酸溶液(20:80),检测波长为403 nm,流速为1.0 ml/min。结果 羟基红花黄色素A在0.816 0~32.64 μg/ml浓度范围内具有良好的线性关系,r=0.999 9,平均加样回收率为101.0%,RSD为1.8%(n=6)。结论 本方法快速、准确、重复性好,可用于伊赫汤中羟基红花黄色素A的含量测定。 |
| 中文关键词:伊赫汤 羟基红花黄色素A 高效液相色谱法 |
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| Determination of hydroxysaffor yellow A in Yihe decoction by HPLC |
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| Abstract:Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction. |
| keywords:Yihe decoction hydroxysaffor yellow A HPLC |
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