| 葛根中黄酮类化学成分的UHPLC-Q-TOFMS分析 |
| 投稿时间：2012-08-15 修订日期：2012-10-29 点此下载全文 |
| 引用本文：周永刚,吴思,毛飞,吴婷婷,吴海棠,柴逸峰.葛根中黄酮类化学成分的UHPLC-Q-TOFMS分析[J].药学实践杂志,2013,31(2):116~119 |
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| 中文摘要:目的 通过超高效液相-单重四级杆-飞行时间质谱(UHPLC-Q-TOFMS)联用技术,对中药葛根的主要化学成分进行有效的鉴定。方法 色谱分离采用ACQUITY UPLC HSS T3(100 mm×2.1 mm, 1.8 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.4 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果 在优化的液质联用条件下,通过单重四级杆-飞行时间质谱鉴定出葛根中18个成分。结论 通过UHPLC-Q-TOFMS联用技术,为鉴定葛根中的化学成分建立起了一种快速、高效的分析方法。 |
| 中文关键词:葛根 超高效液相-单重四级杆-飞行时间质谱 化学成分 |
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| Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS |
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| Abstract:Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOFMS).Methods A ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.4 ml/min. TOFMS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata. |
| keywords:Pueraria lobata UHPLC-Q-TOFMS chemical constituents |
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