| HPLC法测定中药山楂中有效成分绿原酸的含量 |
| 投稿时间：2012-06-01 修订日期：2012-09-12 点此下载全文 |
| 引用本文：梁睿姝,张川,邹豪,金磊.HPLC法测定中药山楂中有效成分绿原酸的含量[J].药学实践杂志,2012,30(6):457~458,477 |
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| 中文摘要:目的 建立测定山楂中绿原酸含量的高效液相色谱(HPLC)法。方法 色谱柱为Sunfire C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.1 mol/L磷酸二氢钠缓冲液(25:75),流速为1.0 ml/min,柱温为28℃,检测器波长为327 nm。结果 绿原酸的质量浓度在7.03~70.3 μg/ml范围内与峰面积值线性关系良好(r2=0.999 6),精密度RSD为0.27%,平均加样回收率为96.92%,RSD为0.92%(n=6)。结论 HPLC法简便易行、结果可靠,可以快速、准确地测定山楂中绿原酸的含量。 |
| 中文关键词:绿原酸 高效液相色谱 山楂 |
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| Determinatin of chlorogenic acid in hawthorn by HPLC |
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| Abstract:Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn. |
| keywords:chlorogenic acid HPLC hawthorn |
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