| 反相高效液相色谱法测定(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸在SD大鼠血浆中的浓度 |
| 投稿时间：2011-04-08 修订日期：2011-11-06 点此下载全文 |
| 引用本文：窦颖辉,王丽,何广卫,吴强,范国荣,杭太俊.反相高效液相色谱法测定(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸在SD大鼠血浆中的浓度[J].药学实践杂志,2012,30(1):42~44,48 |
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| 基金项目:国家"重大新药创制"科技重大专项(2009ZX09301-011). |
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| 中文摘要:目的 建立测定SD大鼠血浆中(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸浓度的反相高效液相色谱(LC-UV)方法,并用于其在SD大鼠体内的药动学研究。方法 采用Diamonsil-C18(2)柱(100 mm×4.6 mm,5 μm),以乙腈:水(含5 mM的醋酸铵,用醋酸调节pH至4.0)=30:70为流动相,测定70只SD大鼠单剂量尾静脉给药15 mg/kg后不同时刻血浆中药物的浓度,并由此计算其在SD大鼠体内的药动学参数。结果 SD大鼠 |
| 中文关键词:(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸 高效液相色谱法 血药浓度 药动学 |
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| Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC |
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| Abstract:Objective To establish a method for determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC. Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v). The single intravenous injection of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics. Results The intra-run and inter-run RSD were less than 15%. The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml , t1/2 was 3.36 h, AUC0~12 was 17.12 μg h/ml, AUC0~∞ was 17.72μg h/ml. Conclusion The method was simple and accurate, which could be used to separation and determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate. |
| keywords:(E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate HPLC plasma concentration Pharmacokinetics |
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