| 薄膜超声法制备槲皮素脂质体研究 |
| 投稿时间：2011-05-27 修订日期：2011-06-27 点此下载全文 |
| 引用本文：陈浩,戴俊东,王玉蓉,祝年青,孙毅坤,王玥,龚卫红.薄膜超声法制备槲皮素脂质体研究[J].药学实践杂志,2012,30(1):32~34 |
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| 基金项目:国家自然科学基金课题(新型抗肿瘤多药耐药长循环脂质体的研究,课题编号:30801549/H2806). |
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| 中文摘要:目的 以粒径和包封率为指标,优选槲皮素脂质体的制备工艺。 方法 以氢化大豆磷脂(HSPC)、胆固醇(CH)为膜材,采用薄膜超声法制备脂质体。通过正交设计优化处方工艺,利用马尔文动态光散射粒径测定仪测定脂质体的粒径,鱼精蛋白沉淀法分离游离药物,HPLC法测定脂质体中槲皮素(QU)的包封率。 结果 最佳处方工艺为:HSPC:CH=3:1、HSPC:QU=20:1、探头超声(600 W)9 min。 结论 薄膜超声法适于实验室条件下制备槲皮素脂质体。 |
| 中文关键词:槲皮素 脂质体 薄膜超声法 鱼精蛋白沉淀法 |
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| Preparation of quercetin liposome by film-ultrasonic technique |
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| Abstract:Objective To study the preparation method of quercetin liposome and screen the optimal technological conditions by the particle sizes and the encapsulation efficiencies of quercetin. Method Liposomes were made of hydrogenated soybean phosphatidylcholine(HSPC) and cholesterol(CH) by film evaporation and probe ultrasonic technique, then orthogonal design was adopted to screen the optimal conditions. The particle sizes were detected by Zetasizer Nano, while the encapsulation efficiency of quercetin (QU) were determined by HPLC after the free drug was separated by protamine sedimentation method. Results The optimal technological conditions of quercetin liposome were as follows: HSPC∶CH=3∶1, HSPC:QU=20:1, the time of probe ultrasonic (600 w) was 9 minutes. Conclusion Film evaporation and probe ultrasonic technique could be suitable for laboratory to prepare quercetin liposome. |
| keywords:quercetin liposome film evaporation and probe ultrasonic technique protamine sedimentation method |
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