| 五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用 |
| 投稿时间：2011-08-11 修订日期：2011-10-14 点此下载全文 |
| 引用本文：陈迁,陈孟莉,叶兆波.五子衍宗复方总多糖对慢性酒精中毒大鼠肝脏的保护作用[J].药学实践杂志,2012,30(1):25~28,31 |
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| 基金项目:国家自然科学基金资助项目(81041030). |
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| 中文摘要:目的 在前期研究工作的基础上,对具有肝脏保护作用的中药五子衍宗复方(WZ)进行进一步的分离,考察WZ复方提取物中总多糖(TPC)对慢性酒精中毒大鼠肝脏的保护作用并探讨其可能的作用机制。 方法 本实验采用改良的Lieber-DeCarli液体酒精饲料喂养致慢性酒精性肝损伤大鼠模型,应用TPC治疗,观察血清生化指标和病理学改变,考察对肝脏氧化应激、脂质过氧化指标和细胞因子TNF-α水平及TNF-α mRNA 表达影响。 结果 TPC能够显著降低升高的肝脏指数和血清ALT、AST水平,减轻肝脏组织病理学改变;减 |
| 中文关键词:五子衍宗丸 多糖 氧化应激 肿瘤坏死因子-α |
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| Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat |
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| Abstract:Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level. |
| keywords:Wuzi-Yanzong pill polysaccharides oxidative stress TNF-α |
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