| HPLC法测定清热消肿口服液中绿原酸的含量 |
| 投稿时间：2010-07-09 修订日期：2011-03-04 点此下载全文 |
| 引用本文：孙学雄,罗晨曲.HPLC法测定清热消肿口服液中绿原酸的含量[J].药学实践杂志,2011,29(4):278~280 |
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| 中文摘要:目的 建立HPLC法测定清热消肿口服液中绿原酸的含量。 方法 采用phenomenex Luna -C18柱(200 mm×4.60 mm, 5 μm),流动相为甲醇-1%冰醋酸(20：80),流速为1.00 ml/min,检测波长326 nm,柱温为30 ℃。 结果 绿原酸进样量在0.163 1~1.087 2 μg范围内与峰面积线性关系良好,r=1.000(n=5),平均回收率为100.40%,RSD为1.20%(n=9)。 结论 该方法简便、准确、可靠、重现性好,可用于该制剂的质量控制。 |
| 中文关键词:清热消肿口服液 绿原酸 HPLC 含量测定 |
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| Determination of Chlorogenic acid in Qingrexiaozhong oral liquid by HPLC |
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| Abstract:Objective To develop a HPLC method for determining the content of Chlorogenic acid in QingRexiaozhong Oral Liquid. Methods The phenomenex Luna-C18(200 mm×4.60 mm ,5 μm)was used ;the mobile phase consisted of water-1%Acetic acid (20：80),the flow rate of 1.00 ml/min,and the detecting wavelength was 326 nm.The column temperature was at 30 ℃. Results There was a good linearity for Chlorogenic Acid in the range of 0.163 1~1.087 2 μg, r=1.000(n=5),and the average recovery rate was 100.40%, RSD=1.20%(n=9). Conclusions The method was simple,accurate,reliable and reproducible,which could be available for the quality control of QingRexiaozhong Oral Liquid |
| keywords:Qingrexiaozhong oral liquid Chlorogenic acid HPLC Content determination |
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