| 总状土木香中倍半萜内酯类同分异构体的鉴定与含量测定 |
| 投稿时间：2010-03-12 修订日期：2010-04-07 点此下载全文 |
| 引用本文：霍炎,史海明,李薇薇,郭澄,李晓波.总状土木香中倍半萜内酯类同分异构体的鉴定与含量测定[J].药学实践杂志,2010,28(5):334~338 |
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| 中文摘要:目的:建立同时测定总状土木香药材中3个倍半萜类同分异构体的高效液相方法。方法:采用Agilent ZorbaxXDB-C18 column(250 mm×4.6 mm,5μm);流动相:55%乙腈和45%水等度洗脱;流速1ml/min;检测波长210 nm。结果:总状土木香药材中3个倍半萜类同分异构体Igalane、异土木香内酯和土木香内酯分别在17.40～1 740.00、21.20～2 120.00和20.40～2 040.00μg/ml的浓度范围内呈良好的线性关系,最低检测限分别为0.087、0.042和0.041μg/ml。重复性、稳定性的RSD均小于2.85%;各成分平均回收率均小于104.43%,RSD<1.20%。结论:该方法>快速简便、准确可靠,各主要化学成分均能达到基线分离,适用于总状土木香药材的质量控制。另外,本实验采用液相半制备得到了两个倍半萜类同分异构体,并对它们的结构进行了鉴定。 |
| 中文关键词:总状土木香 倍半萜内酯 Igalane 异土木香内酯 土木香内酯 高效液相法 同分异构体 |
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| Determination and structural elucidation of sesquiterpene lactones from Inula racemosa |
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| Abstract:Objective To establish a method of HPLC for the simultaneous quantification of three sesquiterpene lactones in Inula racemosa.Methods Agilent Zorbax XDB-C18 column(250 mm×4.6 mm,5 μm) was uesed with a mobile phase consists of 55% acetonitrile and 45% water,at a flow rate of 1.0 ml/min,and detection wavelength of 210 nm.Results Three sesquiterpene lactones in Inula racemosa Hook.f.,namely igalane(1),isoalantolactone(2) and alantolactone(3) has good linear relation in 17.40～1 740.00,21.20～2 120.00,20.40～2 040.00 μg/ml with the lowest detection limit of 0.087,0.042,0.041 μg/ml respectively.The RSD of repeatability and stability were all less than 2.85%.The average recovery rate of there constituents were all less than 104.43% with RSD less than<1.20%.Conclusion The method was rapid,accurate,reliable and could be suitable as a quality control method for I.racemosa.Two minor isomers from I.racemosa were isolated by semi-preparative HPLC which structures were elucidated on the basis of NMR analysis. |
| keywords:Inula racemosa sesquiterpene lactone igalane isoalantolactone alantolactone HPLC isomer |
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