| 分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布 |
| 投稿时间：2009-10-25 点此下载全文 |
| 引用本文：张立超,张永佳,顾伟鹰,王慧菁,时扣荣.分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布[J].药学实践杂志,2009,27(6):417~420 |
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| 基金项目:国家自然科学基金项目(30472103);浦东新区卫生系统学科带头人项目(PWRd2008-07);浦东新区科委科研项目(PKJ2009Y23) |
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| 中文摘要:目的:比较分泌型磷脂酶A2(sPLA2)在人恶性黑素瘤A375细胞、黑素瘤组织与健康人皮肤细胞、组织中的表达和分布。方法:分别采用ELISA法和Western Blot法检测细胞上清液和细胞内sPLA2的表达,免疫组化法考察人皮肤组织中sPLA2的表达和分布。结果:恶性黑素瘤A375细胞上清液的sPLA2浓度为(177.27±13.57)pg/mL,明显高于人表皮HaCaT细胞的(21.42±5.05)pg/mL(P<0.05)和真皮成纤维细胞的(2.75±1.23)pg/mL(P<0.05);其细胞内sPLA2/GAPDH的灰度比值为7.03±1.31,亦明显高于HaCaT细胞的1.45±0.37(P<0.05)和成纤维细胞的0.31±0.11(P<0.05);黑素瘤组织中呈现大量sPLA2阳性细胞和颗粒,而健康人表皮和真皮组织中则未见该阳性细胞和颗粒。结论:sPLA2在人恶性黑素瘤A375细胞和黑素瘤组织中较健康皮肤细胞和组织均有较高表达和分布,提示sPLA2有可能成为皮肤黑素瘤靶向给药的新靶点。 |
| 中文关键词:分泌型磷脂酶A2 黑素瘤 表达 分布 |
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| Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues |
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| Abstract:Objective:To investigate the expression and distribution of secretory phospholipase A2(sPLA2) in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues.Methods:The expression of sPLA2 in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method,respectively.Immunohistochemical method was used to investigate the expression and distribution of sPLA2 in the human skin tissues.Results:The levels of sPLA2 in the clear supernatant of A375 malignant melanoma cells was(177.27±13.57) pg/mL,which was significantly higher(P<0.05) than the group of human epidermal HaCaT cells(21.42±5.05) pg/ml and the group of dermal fibroblast cells(2.75±1.23) pg/mL,respectively.The ratio of sPLA2/GAPDH in the A375 cells was(7.03±1.31),which was also significantly higher(P<0.05) than the group of human epidermal HaCaT cells(1.45±0.37) and the group of dermal fibroblast cells(0.31±0.11),respectively.Many sPLA2 positive cells and granules were presented in the tumor tissues.In contrast,positive cells and granules above were unobserved in the normal human epidermal and dermal tissues.Conclusion:sPLA2 is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues,indicating the sPLA2 maybe a novel trigger for targeted drug delivery to skin melanoma. |
| keywords:secretory phospholipase A2 melanoma expression distribution |
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