| HPLC法测定委陵菜中黄酮类成分的含量 |
| 投稿时间：2008-04-01 点此下载全文 |
| 引用本文：申东月,朴淑娟,林厚文,汤真,张恒弼.HPLC法测定委陵菜中黄酮类成分的含量[J].药学实践杂志,2009,27(1):63~65 |
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| 基金项目:上海-SK研究与发展基金(2003010-t) |
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| 中文摘要:目的:建立HPLC法测定委陵菜中槲皮素、翻白叶苷A和芹菜素含量的方法。方法:色谱柱为ZORB-AXSB-C18色谱柱(4.6 mm×150 mm,5μm);流动相为甲醇-0.3%磷酸水溶液(51:49);检测波长为360 nm;流速为0.9 mL/min。结果:槲皮素、翻白叶苷A和芹菜素的进样量分别在0.72～10.08μg/mL、0.86～12.04μg/mL和0.54～7.56μg/mL范围内与其峰面积呈良好的线性关系,线性系数均为0.999 9,平均回收率分别为95.37%、96.87%、97.66%,RSD分别为1.79%、1.63%、2.08%,药材中三中黄酮的含量分别为100.0μg/g、323.0μg/g和55.0μg/g;结论:该方法快速、简便、可靠,适用于委陵菜中黄酮类成分的含量测定。 |
| 中文关键词:HPLC 委陵菜 槲皮素 翻白叶苷A 芹菜素 |
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| Determination of flavonoids in Potentilla chinensis ser by HPLC |
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| Abstract:Objective: To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser.Methods: ZORBAX SB-C18 column(4.6mm×150mm,5μm) was used,and the mobile phase was methanol-0.3% Phosphoric acid(51:49) with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm.Results:The linear ranges of quercetin,potengriffioside A and apigenin were 0.72～10.08 μg/mL,0.86～12.04μg/mL and 0.54～7.56μg/mL,respectively(r= 0.999 9);the average recovery was 95.37%,96.87% and 97.66%,the RSD was 1.79%,1.63% and 2.08%,respectively;the content of these three flavonoids in the herb was 100μg/g、323μg/g and 55μg/g,respectively.Conclusion:This method is fast,and reliable convenient,which refers to the assay of flavanoids in Potentilla chinensis. |
| keywords:HPLC Potentilla chinensis ser querceitin potengriffioside A apigenin |
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