干姜挥发油提取优化及GC-MS图谱研究

李翔; 吴豪; 朱东亮; 柴逸峰; 娄子洋

干姜挥发油提取优化及GC-MS图谱研究

投稿时间：2008-06-12 点此下载全文

引用本文：李翔,吴豪,朱东亮,柴逸峰,娄子洋.干姜挥发油提取优化及GC-MS图谱研究[J].药学实践杂志,2009,27(1):46~48,50

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作者	单位	E-mail
李翔	第二军医大学药学院药物分析教研室,上海200433 北京军区疾病预防控制中心,北京100042	louziyang@126.com.
吴豪	第二军医大学药学院药物分析教研室,上海200433
朱东亮	第二军医大学药学院药物分析教研室,上海200433
柴逸峰	第二军医大学药学院药物分析教研室,上海200433
娄子洋	第二军医大学药学院分析测试中心,上海200433

中文摘要:目的:优化干姜挥发油提取工艺,同时建立干姜挥发油GC-MS指纹图谱。方法:以挥发油得率为指标,采用正交设计,对加水量、超声时间、浸泡时间和提取时间进行优化,并采集GC-MS图谱。结果:确定加17倍量水,超声35 min,浸泡40 min,再提取8 h的效果最优,挥发油得率分别为1.89%,GC-MS图谱稳定、重复。结论:干姜挥发油经过提取优化可以保证得率,获得的GC-MS图谱稳定可靠,并鉴定出的49种化合物。

中文关键词:干姜挥发油正交设计 GC-MS

Studies on volatile oil extraction by orthogonal design and GC-MS analysis for Rhizoma Zingiberis

Abstract:Objective: To optimize the extraction methods and GC-MS analysis of volatile oil in Rhizoma Zingiberis.Methods: The distilled water volume,ultrasonic time,soaking time and extraction time were optimized by orthogonal design and GC-MS chromatogram was obtained.Results:The yield of volatile oil was 1.89% by orthogonal design.Also,a stable,reliable chromatogram was obtained under optimized GC-MS conditions.Conclusion:The design can be applied for optimizing the extraction condition of volatile oil in Rhizoma Zingiberis and 49 compounds were identified by GC-MS analysis.

keywords:Rhizoma Zingiberis volatile oil orthogonal design GC-MS

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