| 紫外分光光度法测定盐酸奈福泮-β-环糊精包合物的含量 |
| 投稿时间：2007-06-11 点此下载全文 |
| 引用本文：关皓月,张纯,顾伟锋.紫外分光光度法测定盐酸奈福泮-β-环糊精包合物的含量[J].药学实践杂志,2008,(4):291~293 |
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| 中文摘要:目的:建立测定盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的方法。方法:采用紫外分光光度法测定含量,测定波长为267 nm。用乙酸乙酯洗脱未包封的盐酸奈福泮,测定被包封的盐酸奈福泮含量及包封率。结果:盐酸奈福泮检测浓度在0.01～0.4 mg/mL范围内线性关系良好(r=0.9995,n=9);日内RSD =0.17%(n=5),日间RSD=1.82%(n=5);平均加样回收率为100.39%(n=9);测得3批样品的包封率分别为73.28%、70.37%、74.97%。结论:该方法操作简便、结果准确,适用于盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的测定。 |
| 中文关键词:紫外分光光度法 盐酸奈福-β-环糊精包合物 盐酸奈福泮 含量测定 |
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| Determination of nefopam hydrochloride(NFH) in nefopam NFH-β-cyclodextrin inclusion compound by UV spectrophotometry |
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| Abstract:Objective:To establish a method for the content determination of NFH in NFH-13-cyclodextrin inclusion compound. Methods:The drug concentration in NFH-β-eyelodextrin inclusion compound was detected at the wavelength of 267 nm by UV spectro- photometry.The free NFH was eluated by eetoaeetate.The enveloped NFH and its entrapment efficiency were detected by UV spectro- photometry.Results:The linear range of NFH was O.01～0.4 mg/mL (r=0.999 5,n=9);the within day RSD and between-day were 0.17% and 1.82% (u=5),respectively.The average recovery was 100.39% (n=9).The entrapment efficiencies of 3 batches of samples were 73.28%,70.37%,74.97%.Conclusion::The method is simple and reliable,which can be used for the content determi- nation of NFH in NFH-β-cyclodextrin inclusion compound. |
| keywords:UV spectrophotometry NFH-β-cyclodextrin inclusion compound NFH content determination |
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