| 高效液相色谱法测定氯霉素醇溶液中氯霉素的含量 |
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| 引用本文：刘海英,杨宁,黄力,沈振华,苏芳.高效液相色谱法测定氯霉素醇溶液中氯霉素的含量[J].药学实践杂志,2008,(4):289~290,293 |
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| 中文摘要:目的:建立高效液相色谱法测定氯霉素醇溶液中氯霉素的含量。方法:采用高效液相色谱法,色谱柱为ZORBAx 80A Extend-C18（4.6 mm×150 mm,5μm）;以乙腈-0.1%庚烷磺酸钠溶液（25:75）为流动相;检测波长:278 nm;流速:1mL/min;柱温:35℃;进样量:10μL。结果:浓度在24.74～247.4μg/mL（r=0.9999）范围内具有良好线性关系,平均回收率为99.28%,RSD为0.27%（n=9）。结论:方法可靠、简单可行,可用于测定氯霉素醇溶液中氯霉素的含量。 |
| 中文关键词:氯霉素醇溶液 高效液相色谱法 含量测定 |
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| Determination of chloramphenicol alcoholic solution by HPLC |
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| Abstract:Objective:To developed a method for determination of chloramphenicol alcoholic solution.Methods:HPLC method was carried out on a ZORBAX 80A Extend-C18 column（4.6 mm×150 mm,5μm） with UV detection at 278 nm.The mobile phase was acetonitrile-0.1% sodium heptanesulfonate（25:75） with a flow rate of 1.0 mL/min.The column temperture was 35℃and the inject volumes was 10μL.The content of chloramphenicol was calculated by external standardization method.Results:There was a good lin- ear relationship for chloramphenicol within the range of 24.74～247.4μg/mL（r=0.999 9）.The average recovery was 99.28% and RSD was 0.27% （n=9）.Conclusion::TThe method is simple,specific and not interfered by excipients.It is accurate for quality control of chloramphenicol alcoholic solution. |
| keywords:chloramphenicol HPLC alcoholic solution |
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