| 毛细管区带电泳对5种三唑类化合物的手性分离 |
| 投稿时间：2008-03-10 点此下载全文 |
| 引用本文：侯莹,纪松岗,赵亮,孙青龑,柴逸峰.毛细管区带电泳对5种三唑类化合物的手性分离[J].药学实践杂志,2008,(3):214~217 |
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| 中文摘要:目的:考察毛细管区带电泳法(CZE)中各种因素对β-环糊精衍生物手性分离三唑类化合物的影响。方法:采用CZE对5个三唑类化合物的手性分离进行研究。BECKMAN P/ACE System 5 000毛细管电泳仪,紫外检测器。未涂渍熔融石英毛细管50μm×47 cm(有效长度40 cm);正极压力进样50 mbar×3 s,检测波长200 nm。β-环糊精的3种衍生物做为手性添加剂。结果:羧甲醚-β-环糊精为手性选择剂是5个化合物都能达到较好的手性分离,而硫酸酯-β-环糊精和二氟代羧甲醚-β-环糊精对这5个化合物无手性分离能力。在羧甲醚-β-环糊精环境下,优化选择30 mmol/L的NaH2PO4缓冲液含羧甲醚-β-环糊精5 mmol/L,用H3PO4调节pH2.2,温度20℃,电压20 KV,在此条件下5个化合物都能达到良好的手性分离。结论:β-环糊精的种类和缓冲液的pH是影响这5对三唑类对映异构体分离的关键因素。 |
| 中文关键词:毛细管区带电泳 β-环糊精衍生物 三唑类 手性拆分 |
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| Chiral separation of five triazole antifungals by capillary zone electrophoresis |
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| Abstract:Objective: To investigate the factors that influence the chiral separation of triazole antifungals by capillary zone electrophoresis using β-cyclodextrin derivatives as chiral selector.Methods:Analyse were carried out on a P/ACE 5000 capillary electrophoresis system(Beckman,USA) with a UV detector,using an uncoated fused-silica capillary 50 μm × 47 cm with an effective length of 40 cm.Samples were injected into the capillary by 50 mbar pressure for 3 s.The detective wavelength was set at 200 nm.Three β-cyclodextrin derivatives were used as chiral selectors.Results:Satisfactory separation of the five triazole antifungals were achieved when using carboxymethylated-β-cyclodextrin(CM-β-CD) while the sulfobutyl-ether-β-cyclodextrin(SBE-β-CD) and difluoro-carboxymethylated-β-cyclodextrin showed no chiral separation ability to the five triazole antifungals.When using CM-β-CD,the enantiomeric separation of the five compounds and the best results were obtained in 30 mmol/L NaH2PO4 buffer(adjust to pH 2.2 with H3PO4)containing 5 mmol/L CM-β-CD.The voltage was 20 KV and the temperature was 20 ℃.The five triazole compounds were well separated on this condition.Conclusion:The type of β-CD and the pH of buffer is the major factor influencing the separation of the five triazole antifungals. |
| keywords:capillary zone electrophoresis β-cyclodextrin derivative triazole chiral separation |
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