| 反相高效液相色谱法测定罗哌卡因血药浓度 |
| 投稿时间：2006-03-23 点此下载全文 |
| 引用本文：王慧,张国庆.反相高效液相色谱法测定罗哌卡因血药浓度[J].药学实践杂志,2007,(4):238~240 |
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| 中文摘要:目的:建立准确、快速测定罗哌卡因血药浓度的反相高效液相色谱法。方法:血样用乙醚和正己烷提取,布比卡因作内标,氮气吹干,流动相溶解进样。色谱条件:采用AgilentC18色谱柱（4.6 mm×150 mm,5μm）,流动相:0.045 mol/L磷酸二氢钾（pH3.0）-甲醇（55:45）,流速:1.0 mL/min,检测波长:210 nm,柱温:30℃。结果:罗哌卡因在0.250 9～5.018μg/mL范围内线性关系良好（r=0.999 6）,平均回收率102.0%,日内、日间精密度RSD均<15%,符合方法学要求。结论:本法快速、稳定,可用于罗哌卡因血药浓度监测。 |
| 中文关键词:反相高效液相色谱法 罗哌卡因 血药浓度 |
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| Determination of ropivacaine in human plasma by reversed-phase high performance liquid chromatography |
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| Abstract:Objective:To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC.Methods:The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃.Results:The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology.Conclusion:This method is quick and stable for ropivacaine monitoring in clinical research. |
| keywords:RP-HPLC ropivacaine plasma drug concentration |
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