| HPLC法同时测定复方酮康唑凝胶剂中酮康唑和丙酸氯倍他索的含量 |
| 投稿时间：2006-01-25 点此下载全文 |
| 引用本文：张倩,宋洪涛,陈雅娉,康鲁平.HPLC法同时测定复方酮康唑凝胶剂中酮康唑和丙酸氯倍他索的含量[J].药学实践杂志,2006,(6):336~339 |
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| 中文摘要:目的：建立复方酮康唑凝胶剂中酮康唑和丙酸氯倍他索的含量测定方法。方法：采用高效液相色谱法,色谱柱为Hypersil ODS2 C18柱,甲醇-0．2%三乙胺（醋酸调pH至6．9,V：V=75：25）为流动相,流速1．0 mL/min．检测波长239nm。结果：酮康唑在10．08～201．16μg/mL浓度范围内呈良好的线性关系,r=0．9999；丙酸氯倍他索在0．251～5．02μg/mL浓度范围内呈良好的线性关系,r=0．9998。酮康唑的平均回收率为99．59%,RSD为0．77%；丙酸氯倍他索的平均回收率为100．76%,RSD为1．18%。结论：本方法分离度好,简便,准确,适用于复方酮康唑凝胶剂中2组分的同时测定,可作为产品的质量控制方法。 |
| 中文关键词:高效液相色谱法 复方酮康唑凝胶剂 酮康唑 丙酸氯倍他索 |
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| Simultaneous determination of ketoconazole and clobetasol propionate in compound ketoconazole gel by HPLC |
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| Abstract:Objective:To establish a method for simultaneous determination of ketoconazole and clobetasol propionate in compound ketoconazole gel.Methods:HPLC method was developed.Chromatographic conditions included Hypersil ODS2 C18 column and the mo- bile phase was methanol-0.2% triethylamine（adjust with acetic acid to a pH of 6.9,V:V=75:25）.The flow rate was 1.0 mL/ min and the UV detection wavelength was at 239 nm.Results:Ketoconazole had a good linearity（r=0.9999） in the range of 10.08～201.16μg/mL,its average recovery was 99.59%（RSD=0.77%）.Clobetasol propionate had a good linearity（r=0.9998） in the range of 0.251～5.02μg/mL,its average recovery was 100.76%（RSD=1.18%）.Conclusion:The method was simple, sensitive and accurate for simultaneous determination of the two constituents in compound ketoconazole gel. |
| keywords:HPLC compound ketoconazole gel ketoconazole clobetasol propionate |
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