| 毛细管电泳和高效液相作氨氯地平异构体分离分析研究 |
| 投稿时间：2006-01-15 点此下载全文 |
| 引用本文：胡建国,乐键,刘长海,陈桂良.毛细管电泳和高效液相作氨氯地平异构体分离分析研究[J].药学实践杂志,2006,(5):289~291 |
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| 中文摘要:目的：毛细管电泳和高效液相作氨氯地平异构体分离分析研究。方法：毛细管电泳采用未涂渍熔融石英毛细管75μm×68cm(有效长度50cm)；优化选择浓度为100mmol/L的Tris缓冲液, 含有1．0%羟丙基-β-CD(用磷酸调节pH至3．0)；分离电压18kV；温度15℃。高效液相色谱柱为ULTRON ES-OVM手性柱, 流动相：乙腈-0．02mol/L磷酸二氢钠溶液(pH 7．0)(20∶80)；流速1．0ml/min。结果：氨氯地平对映异构体分离良好。结论：该方法简单可靠、专属性强。 |
| 中文关键词:毛细管电泳 氨氯地平 手性 |
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| Separation of Enantiomer of Amlodipine by CE and HPLC |
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| Abstract:Objective:Separation of enantiomer of amlodipine by CE and HPLC.Methods:Different separation conditions such as buffer pH, the concentration of the chiral selector, buffer concentrations, temperature and applied voltage, etc.were studied and op-timized.The separation was finally conducted in 100mM orthophosphoric acid-Tris buffer (pH3.0) with 1.0% (w/w) HP-β-CD.The applied voltage was 16kV and the temperature 15℃.Results:Both CE and HPLC could give enantiomer of amlodipine resolution for adrenaline.Conclusion:The method proved to be specific and accurate |
| keywords:capillary electrophoresis amlodipine chiral |
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