| 柱前衍生化HPLC法测定人纤维蛋白原中精氨酸的含量 |
| 投稿时间：2005-12-16 点此下载全文 |
| 引用本文：赵艳红,王强,陈兆梅.柱前衍生化HPLC法测定人纤维蛋白原中精氨酸的含量[J].药学实践杂志,2006,(5):270~271 |
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| 中文摘要:目的：建立人纤维蛋白原中精氨酸的含量测定方法。方法：以丙氨酸为内标, 2, 4-二硝基氟苯(DN-FB)为柱前衍生剂, 50%乙腈-0．05mol/L醋酸盐缓冲液(35∶65)为流动相, 紫外362nm处检测。结果：精氨酸在0．16～0．80mg/mL浓度范围内线性关系良好(r=0．9998), 平均空白加样回收率为102．4%, RSD为1．4%。结论：方法准确、简便, 费用低, 可用于人纤维蛋白原中精氨酸含量的控制。 |
| 中文关键词:精氨酸 丙氨酸 高效液相色谱法 衍生化 2, 4-二硝基氟苯 |
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| Determination of arginine in human fibrinogen by HPLC with precolumn derivatization |
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| Abstract:Objective:To develop a method for determination of arginine in human fibrinogen.Methods:The sample was mixed with alanine as the internal standard, derived by 2, 4-dinitrofluorobenzene.The mobil phase was composed of 50% acetonitrile-0.05 mol/L acetate buffer(35∶65).The UV detection wavelength was 362nm.Results:There was a good linear relationship with the range of 0.16～0.06mg/mL(r=0.9998), average recovery of arginine was 102.4%, RSD was 1.4%.Conclusion:This method is accu-rate, simple, cheap, and can be used for determination of arginine in human fibrinogen. |
| keywords:arginine alanine, HPLC derivation 2, 4-dinitrofluorobenzene |
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