| 反相高效液相色谱法测定复方鼻用软膏中磺胺嘧啶含量 |
| 投稿时间：2003-12-22 点此下载全文 |
| 引用本文：吴洪文,吴敏.反相高效液相色谱法测定复方鼻用软膏中磺胺嘧啶含量[J].药学实践杂志,2004,(6):333~335 |
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| 中文摘要:目的 建立复方鼻用软膏中磺胺嘧啶的含量测定方法。方法 反相高效液相法,日本岛津Shim-pack VP-ODS柱(250L×4.6),国产C18保护柱,流动相:甲醇-水-冰醋酸(40:60:0.1),检测波长:270nm,流速0.6mL/min。结果 在11.26～123.82μg/mL范围内成线性关系,Y=144647X-41558,r=0.9999,平均回收率为100.2±1.5%,RSD为1.4%。结论 本方法简单、准确、快速,可用于本制剂磺胺嘧啶的快速定量分析。 |
| 中文关键词:反相高效液相色谱法 磺胺嘧啶 复方鼻用软膏 |
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| Determination of sulfadiazine in compound nasal ointment by RP-HPLC |
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| Abstract:Objective To establish a method for determination of the content of sulfadiazine in compound nasal ointment.Methods RP-HPLC was adopted with Shim-pack VP-ODS(250L×4.6) column as analytical column, methanol-water-aceti-cacid (40:60:0.1) as mobile phase.The detection wavelength was 270nm.The flow rate was 0.6mL/min. Results The linear ranges of sulfadiazine were 11.26-123.82μg/mL,Y = 144647X-41558,r =0.9999,the average recovery was (100.2±1.5)%,RSD was 1.4%(n=6).Conclusion The method is simple, rapid, accurate and suitable for the determination of sulfadiazine in compound nasal ointment. |
| keywords:RP-HPLC sulfadiazine compound nasal ointment |
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