HPLC测定左金丸中吴茱萸次碱含量

潘敏翔; 刘昌叶; 常华; 赵淼; 姜韧

HPLC测定左金丸中吴茱萸次碱含量

投稿时间：2002-03-04 点此下载全文

引用本文：潘敏翔,刘昌叶,常华,赵淼,姜韧.HPLC测定左金丸中吴茱萸次碱含量[J].药学实践杂志,2002,(4):236~237

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作者	单位
潘敏翔	解放军306医院药剂科北京 100101
刘昌叶	解放军306医院药剂科北京 100101
常华	解放军306医院药剂科北京 100101
赵淼	解放军306医院药剂科北京 100101
姜韧	解放军306医院药剂科北京 100101

中文摘要:目的建立高效液相色谱法测定左金丸中吴茱萸次碱含量的方法。方法采用高效液相色谱法。IntersilC₁₈分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相为乙腈—10%乙腈(50:50),流速1ml·min^-1,检测波长345nm。结果吴茱萸次碱的理论板数为2100。回归方程Y=-0.2197+0.0000003484X(r=0.9999),线性范围12～60μm·ml^-1。平均回收率为96.9%(n=5),RSD 3.7%,最低检出浓度为0.lμg·ml^-1。结论方法简便,结果准确。

中文关键词:高效液相色谱法左金丸吴茱萸次碱

Detemination of rutaecarpine In Zuojin Wan by HPLC

Abstract:OBJECTIVE A quantitative method was developed for the determination of rutaecarpine in Zuojin Wan by HPLC. METHOD The chromatographic conditions include column Intersil C₁₈, mobile phase:acetonitrile-10% acetonitrile (50:50), flow rate 1 ml·min^-1, wavelength 345nm. RESULTS The number of theoretical plates caculated by rutaecarpine peak was 2100, The regression equation of rutaecarpine was Y=-0.2197+0.000000349X(r=0.9999) and the liner range was 12-60μg·ml^-1. The average recovery of rutaecarpine was 96.9% (n=5),RSD 3.7%. The measurable lowest limit of rutaecarpine was 0.1μg·ml^-. CONCLUSION The method is sensitive, rapid and accurate.

keywords:HPLC Zuojin wan rutaecarpine

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