| RP-HPLC法测定优赛清栓剂中盐酸黄连素含量 |
| 投稿时间：2000-07-08 点此下载全文 |
| 引用本文：刘有平,杨振华,曹延军.RP-HPLC法测定优赛清栓剂中盐酸黄连素含量[J].药学实践杂志,2001,(1):27~28,30 |
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| 中文摘要:目的 建立反相高效液相色谱法测定优赛清栓剂中盐酸黄连素含量的方法。方法 采用shim-packCLC-ODS(4.5mm×150mm)色谱柱,以H3PO4(0.01mol/L)MeOH(AR)(65:35)为流动相,流速为10ml/min,检测波长为279nm。结果 线性范围为3～100μg/ml,回归系数为r =0.9999(n=6),测得盐酸黄连素的回收率为99.4 %,相对标准偏差为1.49%。结论 本方法简便、准确、可靠,适用于常规分析。 |
| 中文关键词:RP-HPLC 优赛清栓剂 盐酸黄连素 |
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| RP-HPLC determination of berberine hydrochloride in You Sai Qing suppository |
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| Abstract:OBJECTIVE To study resersed phase high performance liquid chromatographic method for the determination of berberine hydrochloride (Ber) in You Sai Qing suppository.METHODS Ber was chromatographed on a shim pack CLG-ODS column with a mobile phase consisting of H3PO4 (0.01mol/L):MeOH(AR)(65:35).The flow rate was 1.0ml/min and the monitoring wavelength is 279nm. RESULTS The calhration curve was linear in the range of 3～100μg/ml.The recovery was 99.4%,RSD was 1.49%.CONCLUSION The methed is simple, accurate and relief and Can be used to determine the Ber. |
| keywords:RP-HPLC You Sai Qing suppository berberine hydrochloride |
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