| 富锗人参细胞的培养及含量测定 |
| 投稿时间：2000-08-28 点此下载全文 |
| 引用本文：刘峻,丁家宜.富锗人参细胞的培养及含量测定[J].药学实践杂志,2000,(5):288~289 |
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| 中文摘要:目的 通过在培养基里加入二氧化锗,培养获得富锗人参细胞(GSC),并对其进行组分分离及探讨锗的分布情况。方法 应用胶束增溶分光光度法测定富锗GSC中锗的含量。结果 在5ppm的富锗培养基上培养的GSC中锗的转化率最高(11.18%).锗在GSC中可能主要以和蛋白质及多糖结合的形式而存在,且单位蛋白质结合的锗的量大于单位糖结合的锗的量。结论 GSC对GeO2转化率随GeO2的培养浓度增加而减少,证明GSC对GeO2有一定的吸收度,GSC可望成为有机锗转化的合适载体。 |
| 中文关键词:锗 胶束增溶分光光度法 人参细胞(GSC) |
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| Determination of Germanium in Enriched Germanium Panax ginseng Cells(GSC) by Micellar Solubilizing Spectrophotometry |
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| Abstract:OBJECTIVE To obtain enriched germanium ginseng cell(GSC)and to detect the.Distribution of germanium in GSC.METHODS Enriched GSC was examined by micellar solubilizing spectrophotometry.RESULTS GSC contains the highest(11.18%)germanium in 5ppm enriched germanium MS cultrue medium. Germanium largely conjugated with protein and polysaccharide and it mostly conjugated with protein. CONCLUSION GeO2 transforming ratio decrease along with increase the concentration of germanium MS culture medium, which proved GSC can absorb germanium and it s a bearer for GeO2 transforming organic germanium. |
| keywords:germanium micellar solubilizing spectrophotometry panax ginseng cell(GSC) |
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